Journal of the Graduate Research Center


Diamond (1954) described a technique for the detection of trypanosomes in geese. We used a similar technique in the search for trypanosomes in each of 64 mourning doves (Zenaidura macroura) collected on September 1-2, 1960, 4 miles west of Celina, Denton County, Texas. The condylar surfaces of the femur, sterilized with alcohol, were removed with sterile scissors, and the marrow, forced from the femur with a hemostat, was collected with a sterile nichrome wire. The inoculum was incubated in the blood agar, broth overlay medium described by Diamond. One sample of the culture was stained on the 6th day of incubation, and a second sample was stained on the 14th day.

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