Contributor

Juan Pablo Oviedo, Y.M. Nuwan D.Y. Bandara, Xin Peng, Longsheng Xia, Qingxiao Wang, Kevin Garcia, Jinguo Wang, Moon Kim, Min Jun Kim

Abstract

Accomplishing slow translocation speed with high sensitivity has been the greatest mission for solid-state nanopore (SSN) to electrically detect nucleobases in single-stranded DNA (ssDNA). In this study, a method to detect nucleobases in ssDNA using a SSN is introduced by considerably slowing down the translocation speed and effectively increasing its sensitivity. The ultra-thin titanium dioxide (TiO2) coated hexagonal boron nitride (h-BN) nanopore was fabricated, along with an ionic-liquid [bmim][PF6]/2.0 M KCl aqueous (cis/trans) interfacial system, to increase both the spatial and the temporal resolutions. As the ssDNA molecules entered the nanopore, a brief surge of electrical conductivity was observed, which was followed by multiple resistive pulses from nucleobases during the translocation of ssDNA. The continuous detection of nucleobases using a SSN is a novel achievement, enabled by water molecules bound to ssDNA which increases molecular conductivity of ssDNA and amplifies electrical signals during the translocation event. Along with the experiment, computational simulations using COMSOL are presented to explain the pivotal role of water molecules bound to ssDNA for the detection of nucleobases using a SSN.

Degree Date

Winter 12-19-2020

Document Type

Dissertation

Degree Name

Ph.D.

Department

Applied Science

Advisor

Dr. MinJun Kim

Subject Area

Bioengineering and Biomedical Engineering, Chemical Engineering, Mechanical Engineering

Number of Pages

100

Format

.pdf

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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