Subject Area
Cell Biology, Molecular Biology, Neuroscience
Abstract
Neurodegenerative diseases have a large negative impact to human society. Symptoms of neurodegenerative diseases includes memory loss, impaired recognition, motor dysfunction due to dysregulated neuronal loss in different brain regions. However, the neurobiological basis of these brain diseases is not fully understood and there are no cures or effective treatments. Polyglutamine (Poly-Q) disorders is a class of neurodegenerative diseases that are caused by polyglutamine expansion within the protein coding regions of specific genes. Huntington’s disease (HD), Spinal Cerebellar Ataxia Type 1 (SCA1) and Spinal Cerebellar Ataxia Type 3 (SCA3) are three common diseases among Poly-Q disorders. To better understand the neurobiology of these disorders and further to develop a potential treatment, I focused my dissertation research on p65 which was reported by our laboratory to prevent low-potassium (LK) condition induced program cell death in cerebellar granule neurons (CGN). I discovered that p65 was protective against toxicities in disease models of these disorders. p65 was also showed to prevent neuronal death under oxidative stress in cortical neurons. The protective effect of p65 depended on the Serine at aa276. Mutating p65 S276 to a non-phosphorylatable Alanine (S276A) abrogated the protection of p65 in models of Huntington’s disease and conditions of LK; however, mutating to phosphomimetic Aspartate (S276D) completely protected neurons.
Degree Date
Summer 8-2020
Document Type
Dissertation
Degree Name
Ph.D.
Department
Biological Sciences
Advisor
Dr. Jonathan E. Ploski
Second Advisor
Dr. William C. Orr
Third Advisor
Dr. Richard S. Jones
Fourth Advisor
Dr. Robert Harrod
Number of Pages
48
Format
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Recommended Citation
Zhang, Yiyu, "RelA/p65 Blocks Histone Deacetylase-3 Neurotoxicity and Protects Neurons Against Neuronal Death Induced by PolyQ-Expanded Huntingtin and Ataxin-1 in a p65 Phospho S276 Dependent Manner" (2020). Biological Sciences Theses and Dissertations. 9.
https://scholar.smu.edu/hum_sci_biologicalsciences_etds/9